mouse amyloid beta 42 elisa kit  (Thermo Fisher)

Journal: iScience

Article Title: FOXE1 promotes the progression of pulp inflammation by activating PANoptosis in dental pulp cells

doi: 10.1016/j.isci.2026.115204

Figure Lengend Snippet: Identification and validation of FOXE1 as a key transcription factor that regulates PANoptosis in DPCs (A) Differentially expressed gene analysis was performed between the PANoptotic DPC cluster (cluster 1) and other DPC clusters (clusters 0, 2, 4, 5, and 6) and between cluster 1 and all remaining clusters. The candidate regulators were selected from the overlapping DEGs between these comparisons. (B) The top five candidate regulators were identified after comparison. (C) siRNA targeting FOXE1 (20 μM) was transfected into DPCs with Lipofectamine 2000 for 24 h. The knockdown efficiency was validated by qRT-PCR ( n = 3 experiments in each group) and Western blot. (D–H) Dental pulp cells were pretreated with siRNAs (20 μM) targeting candidate regulators for 24 h, followed by infection with S.m. (MOI = 100) and F.n. (MOI = 100) for 8 h. (D) Cell death was quantified by the LDH release assays ( n = 5 experiments in each group). (E, also see in ) Cell death was assessed by PI staining ( n = 4 experiments in each group). (F) Treated cells were analyzed by flow cytometry. (G) PANoptotic DPCs were quantified by determining the percentages of PI + /annexin V + cells ( n = 3 experiments in each group). (H) Western blot analysis was used to assess the expression of cleaved caspase-3 p17, cleaved GSDMD NT and p -MLKL. (I) Immunofluorescence staining of PANoptotic markers in si FOXE1 -and siCON-transfected DPCs. Scale bars, 10 μm. (J) Quantification of PANoptotic DPCs in I ( n = 5 samples in each group). Data are represented as mean ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 by Student’s t test (C, G, and J) or by one-way ANOVA (D and E).

Article Snippet: The following primary antibodies were used in this study: human and mouse vimentin (1:200, #ab92547, Abcam), human cleaved caspase-3 (1:100, #9664, CST), human cleaved GSDMD (1:100, #36425, CST), human p-MLKL (1:100, #PA5-105678, Invitrogen), mouse cleaved caspase-3 (1:100, #BF0711, Affinity), mouse cleaved GSDMD (1:100, #ab255603, Abcam), mouse p-MLKL (1:100, #ab196436, Abcam) and mouse FOXE1 (1:50, #55363-1-AP, Proteintech).

Techniques: Biomarker Discovery, Comparison, Transfection, Knockdown, Quantitative RT-PCR, Western Blot, Infection, Staining, Flow Cytometry, Expressing, Immunofluorescence

Journal: iScience

Article Title: FOXE1 promotes the progression of pulp inflammation by activating PANoptosis in dental pulp cells

doi: 10.1016/j.isci.2026.115204

Figure Lengend Snippet: Knockdown of Foxe1 in mouse dental pulp inhibited PANoptosis and attenuated inflammatory responses (A) Schematic diagram of the Adv-mediated knockdown experiments in mouse dental pulp tissue. (B) The efficiency of Adv-sh Foxe1 -mediated knockdown was assessed by immunofluorescence. Scale bars, 100 μm. (C) Representative images of inflammatory responses in dental pulp tissue from control and Foxe1 knockdown mice. The black triangle indicates inflammatory infiltration. Scale bars, 100 μm. (D) Evaluation of inflammatory scores in control and Foxe1 knockdown mice ( n = 5 mice in each group). (E) The expression levels of PANoptotic markers were assessed by Western blot. Protein was extracted from mouse dental pulp tissue from the control ( n = 6 mice), and Foxe1 knockdown ( n = 5 mice) groups, and proteins from each group were pooled to form one biological sample. (F) Multiple immunofluorescence staining of PANoptotic DPCs in the dental pulp tissue of control and Foxe1 knockdown mice. Scale bars, 10 μm. (G) Quantification of PANoptotic DPCs in F ( n = 4 samples in each group). Data are represented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01 by Student’s t test (D) or Welch’s t test (G).

Article Snippet: The following primary antibodies were used in this study: human and mouse vimentin (1:200, #ab92547, Abcam), human cleaved caspase-3 (1:100, #9664, CST), human cleaved GSDMD (1:100, #36425, CST), human p-MLKL (1:100, #PA5-105678, Invitrogen), mouse cleaved caspase-3 (1:100, #BF0711, Affinity), mouse cleaved GSDMD (1:100, #ab255603, Abcam), mouse p-MLKL (1:100, #ab196436, Abcam) and mouse FOXE1 (1:50, #55363-1-AP, Proteintech).

Techniques: Knockdown, Immunofluorescence, Control, Expressing, Western Blot, Staining